Optimal excitation and emission wavelengths to analyze amino acids and optimize neurotransmitters quantification using precolumn OPA-derivatization by HPLC
نویسندگان
چکیده
منابع مشابه
Analysis of Plasma Amino Acids Using RP-HPLC and Pre-Column Derivatization with OPA/3-MPA
ABSTRACT Background and Objective: Measurement of amino acids is an important tool for metabolic studies and evaluation of patients’ clinical condition. The aim of this study was to analyze the plasma amino acids using reverse phase high performance liquid chromatography techniques (RP-HPLC) with pre-column derivatization by o-phthalaldehyde (OPA)...
متن کاملanalysis of plasma amino acids using rp-hplc and pre-column derivatization with opa/3-mpa
abstract background and objective: measurement of amino acids is an important tool for metabolic studies and evaluation of patients’ clinical condition. the aim of this study was to analyze the plasma amino acids using reverse phase high performance liquid chromatography techniques (rp-hplc) with pre-column derivatization by o-phthalaldehyde (opa) in combination with 3- mercaptopropionic...
متن کاملApplication Note ► Determination of Amino acids by UHPLC with automated OPA- Derivatization by the Autosampler
Category Bio Analysis Matrix Method UHPLC
متن کاملApplication Note ► Determination of Amino acids by UHPLC with automated OPA- Derivatization by the Autosampler
Category Bio Analysis Matrix Method UHPLC
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A new, simple normal phase high performance liquid chromatographic method was developed for the analysis of aspartame in tabletop sweeteners. The method is based on the derivatization of aspartame with 2, 4-dinitrofluorobenzene followed by detection at 332 nm. Separation was carried on silica column with hexane: ethyl acetate (60: 40, V/V) as mobile phase (pH was adjusted to 4.5 using 1% acetic...
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ژورنال
عنوان ژورنال: Amino Acids
سال: 2015
ISSN: 0939-4451,1438-2199
DOI: 10.1007/s00726-015-1925-1